Journal: Journal of controlled release : official journal of the Controlled Release Society

Article Title: Microparticles synthesized from itaconate polyesters enable metabolite delivery and elicit immunoregulatory outcomes

doi: 10.1016/j.jconrel.2026.115115

Figure Lengend Snippet: A) BMDMs pre-treated for 16h under indicated conditions, followed by LPS+ATP stimulation for 24h before supernatants were collected for analysis of IL-1β secretion, measured via ELISA. B) Pre-treated BMDMs stimulated with poly(I:C) for 24h. IFNβ secretion was assayed via ELISA. C) Quantification of Gclm, Nqo1, and Hmox1 gene expression analysis in BMDMs following 16h pre-treatment and 24h macrophage polarization, expressed as relative to stimulated, untreated cells (LPS only). D) IFNβ secretion by BMDCs pretreated for 16h as indicated, followed by poly(I:C) stimulation for 24h. N=3-4, data are representative of two or more experiments (n=2-3). Treatments = 10ug pAA or pITA MPs, 0.25mM 4-OI or DMI. Error bars represent mean ± SD. *p<0.05, **p<0.01, ***p<0/001 by one-way ANOVA using Tukey-Kramer multiple comparisons test.

Article Snippet: For assays, 100,000 BMDC or BMDMs were seeded in 96-well flat bottom tissue culture plates for 1.5hrs, followed by treatment with 10ug (unless otherwise indicated) MPs for 6-16hrs before 100ng/mL LPS (for IL-6 secretion), 100ng/mL LPS (3hr) followed by 5mM ATP (for IL-1β secretion), or 20ug/mL high molecular weight poly(I:C) (Invivogen) (for IFNβ secretion).

Techniques: Analysis, Enzyme-linked Immunosorbent Assay, Gene Expression

Journal: PLOS Pathogens

Article Title: PRMT3 restricts porcine epidemic diarrhea virus replication by disrupting the interaction between VAPA and the viral nucleocapsid protein

doi: 10.1371/journal.ppat.1014599

Figure Lengend Snippet: (A) WT and PRMT3 KO LLC-PK1 cells were treated with poly(I:C) for 24 h. mRNA levels of ISG15 and ISG56 were quantified by RT-qPCR. (B and C) WT and PRMT3 KO LLC-PK1 cells were infected with PRV-BarthaK61 (MOI = 0.001) (B) or PDCoV-CZ2020 (MOI = 0.1) (C) for 24 h. mRNA levels of ISG15 and ISG56 were quantified by RT-qPCR. (D and E) WT and PRMT3 KO LLC-PK1 cells were infected with PRV-BarthaK61 (MOI = 0.001) for 24 h. PRV genomic copy number was measured by absolute RT-qPCR (D), and viral titer was determined by TCID₅₀ assay (E). (F and G) WT and PRMT3 KO cells were infected with PDCoV-CZ2020 (MOI = 0.1) for 24 and 48 h. Viral RNA was quantified by RT-qPCR (F), and viral titer was determined by TCID₅₀ assay (G). (H) WT and PRMT3 KO LLC-PK1 cells were infected with PRV-BarthaK61 or PRV-EGFP (MOI = 0.001) for 24 h. CPE and GFP fluorescence were observed. (I) WT and PRMT3 KO LLC-PK1 cells were infected with PDCoV-CZ2020 (MOI = 0.1) for 24 h, then fixed and stained with crystal violet to visualize CPE. (J and K) WT and PRMT3 KO cells were infected with PEDV LW/L (J) or TGEV-SD/L (K) for 24 h. mRNA levels of ISG15 and ISG56 were quantified by RT-qPCR. (L and M) WT and PRMT3 KO LLC-PK1 cells were infected with TGEV-SD/L (MOI = 0.01) for 24 h. Viral RNA was quantified by RT-qPCR (L), and viral titer was determined by TCID₅₀ assay (M). All data are representative of three independent experiments and are expressed as mean ± standard error of the mean (SEM). P-values were calculated using unpaired Student’s t-tests or one-way analysis of variance (ANOVA). Statistical significance is denoted as ns: not significant (P > 0.05), *P < 0.05, **P < 0.01, and ****P < 0.0001. All Western blot results are representative of at least three independent experiments.

Article Snippet: Poly(I:C) HMW (tlrl-pic) was purchased from InvivoGen.

Techniques: Quantitative RT-PCR, Infection, Fluorescence, Staining, Western Blot